UVAS SWAT

Academic Qualifications

(2025)
ISOLATION, BIOCHEMICAL CHARACTERIZATION AND MOLECULAR TYPING OF SALMONELLA ENTERICA FROM FRESH MEAT. PUBLIC HEALTH PERSPECTIVE
The Salmonella enterica is associated with foodborne infections throughout the world, making its presence in fresh meat a serious public health concern. To determine the Potential risk to human health, this study aimed to Isolate, biochemically Characterize, and molecularly type S. enterica strains from fresh meat samples. Four fresh meat samples of pork, chicken, and beef were Collected from different markets and examined microbiologically. Isolation was carried out using selective enrichment and differential media, followed by Gram staining and biochemical confirmation. Molecular identification was performed using 16S rRNA sequencing, and chromatogram analysis confirmed the species identity. PCR was used to detect key antibiotic resistance and virulence genes (invA, hilA, and stn). Phylogenetic tree analysis and multilocus sequence typing (MLST) provided insight into genetic diversity and epidemiological linkages of the isolates. This study highlights the importance of regular monitoring of meat for S. enterica to mitigate public health risks. Our results emphasize the need for improved food safety protocols and antimicrobial stewardship. The integration of biochemical and molecular tools offers a robust framework for the surveillance and control of Salmonella in the food chain.description...
Rehman et al., 2025,SOLATION, BIOCHEMICAL CHARACTERIZATION AND MOLECULAR TYPING OF SALMONELLA ENTERICA FROM FRESH MEAT. PUBLIC HEALTH PERSPECTIVE, Frontier in medical and health research,Volume 3, Issue 3, 2025
Authors: Zubair Rehman,Muhammad Khurshaid,Shafiq Ur Rahman,Zanib Qasim,Sania,Ubaid Zakir,Muhammad Usman Shoukat,Noor ZadaKhan,Yasar Aziz,Waseem Sajjad,Mudasir Ahmad,Fahim Ullah | Journal Name: Frontier in Medical and Health Research | Volume, Issue and Pages: Volume 3, Issue 3, 2025 | Publisher: Zubair Rehman
(2025)
MOLECULAR SCREENING OF HEPATITIS B IN YOUNG ADULTS OF DISTRICT ABBOTTABAD AND MANSEHRA
Human hepatitis B is the inflammation of liver cells, caused by hepatitis B virus. It is a serious liver disease that occurs worldwide among people of all ages. It is a highly infectious disease and can result in severe illness, liver damage, and cirrhosis besides increasing the risk for hepatocellular carcinoma (HCC). The disease ranges in severity from a mild illness to a serious, lifelong illness. A cross sectional study was conducted during September 2017 to March 2018 to determine the frequency distribution of active hepatitis B virus (HBV) infection in apparently healthy young residents (5-24 years) of districts Abbottabad and Mansehra (KPK), Pakistan. A total of 353 samples were collected from various schools and colleges of districts Abbottabad and Mansehra. Initially all the individuals were screened for HBV using ICT technique. ICT positive individuals were then subjected to ELISA for the detection of anti-HBV antibodies. The ELISA positive samples were then subjected to polymerase chain reaction (PCR) for further screening (detection of HBV-DNA). Out of 353 samples, 8 (2.26%) cases were found to be positive in the young population of district Mansehra, while 5 (1.41%) cases were found positive in district Abbottabad by ICT technique. By ELISA 6 (1.69%) cases were found positive in district Mansehra whereas 2 (0.56%) cases were noticed positive in district Abbottabad. Subsequent experiments, i.e., PCR showed only one case (0.699%) in district Abbottabad while 02 (0.96%) positive cases in district Mansehra. High Prevalence rate was detected both in female (2.77%) and male (2.39%) population of district Mansehra.Write here the description...
Sayed wajid et al., 2025,MOLECULAR SCREENING OF HEPATITIS B IN YOUNG ADULTS OF DISTRICT ABBOTTABAD AND MANSEHRA,Journal of Medical and Health Science review,
Authors: Syed Wajid Ali Shah1 , Jasia Abbasi2 , Wajiha Waheed3 , Jaweria Safeer4 , Rafi Ullah5 , Noor Zada Khan6 , Muhammad Khurshaid 7 , Iftikhar Alam8 , Fahim Ullah9* , Zubair Rehman | Journal Name: Journal of Health Science and Review | Volume, Issue and Pages: Volume 2, Issue 2, 2025 | Publisher: Sayed Wajid Ali sha
Link http://dx.doi.org/10.62019/0bvdym95